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2.11 Reduced glutathione quantification GSH quantification was performed using established Ellman Assay, where GSH reacts specifically with the Ellman’s reagent (DTNB) to yield 2-nitro-5-mercapto-benzoic acid (TNB), which produces a concentration-dependent yellow chromophore quantifiable by spectrophotometry (Vuolo et al., 2022)
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