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Western blotting Cells were lysed on ice with modified RIPA buffer containing 50 mM Tris–HCl (pH 7.5), 150 mM NaCl, 2 mM EDTA, 1% (v/v) Triton X-100, 0.5% (w/v) sodium deoxycholate, 0.1% (w/v) SDS, 5 mM NaF, 1 mM Na 3 VO 4 , and a protease inhibitor cocktail (GeneDEPOT

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