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Enzymatic reaction The lysate that contained 5 pmol of HIV-PR or HIV-PR mutant was directly used for the enzymatic reaction (50 μl), which was performed at 37 °C for 4 h in 50 mM sodium acetate buffer (pH 5.5) containing 200 μM [Ac]-SGIFLETSLE, 200 μM [Ac]-ARVLFEAM and 800 μM [Ac]-KSGVFVQNGL as multi-substrates
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doi: Greenberger S, Harats D, Salameh F, et al
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